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congenital cmv  (ATCC)


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    Structured Review

    ATCC congenital cmv
    Congenital Cmv, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/congenital+cmv/Cucumber+mosaic+virus+antiserum/pm40840474-49-5-45
    Average 90 stars, based on 10 article reviews
    congenital cmv - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Immunological profile of pregnant women with preconception immunity with or without vertical transmission of human cytomegalovirus to the fetus: a retrospective observational study.
    Article Snippet: .. Serum neutralising activity of the congenital CMV and no-congenital CMV groups were compared with those of primary infection group 1 at 1, 2, 3, 6, and 12months after the onset of infection.For theneutralisation assay, we used VR1814, a clinical isolate, for epithelial cells, and AD169 (American Type Culture Collection [ATCC], Manassas, VA, USA), a reference laboratory-adapted strain, for fibroblast cells. .. After 1 h of incubation at 37◦C and 5%CO2, 50μLperwell of virus (100 focus-formingunits per well) and human serum antibodymixtures were added onto monolayers of epithelial cells (ARPE-19, ATCC) or human embryonic lung fibroblast cells (human embryonic lung fibroblast [HELF], isolated in-house) in 96 well plates, and centrifuged at 600× g for 30min.After incubation for 48 h at 37◦C and 5% CO2, cells were fixed and stained using a p72-specific murine monoclonal antibody (produced in-house).

    Infection:

    Article Title: Immunological profile of pregnant women with preconception immunity with or without vertical transmission of human cytomegalovirus to the fetus: a retrospective observational study.
    Article Snippet: .. Serum neutralising activity of the congenital CMV and no-congenital CMV groups were compared with those of primary infection group 1 at 1, 2, 3, 6, and 12months after the onset of infection.For theneutralisation assay, we used VR1814, a clinical isolate, for epithelial cells, and AD169 (American Type Culture Collection [ATCC], Manassas, VA, USA), a reference laboratory-adapted strain, for fibroblast cells. .. After 1 h of incubation at 37◦C and 5%CO2, 50μLperwell of virus (100 focus-formingunits per well) and human serum antibodymixtures were added onto monolayers of epithelial cells (ARPE-19, ATCC) or human embryonic lung fibroblast cells (human embryonic lung fibroblast [HELF], isolated in-house) in 96 well plates, and centrifuged at 600× g for 30min.After incubation for 48 h at 37◦C and 5% CO2, cells were fixed and stained using a p72-specific murine monoclonal antibody (produced in-house).



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